Clc sequence viewer 8 download6/8/2023 ![]() ![]() Globally, >30% of all rotavirus-related deaths occurs in India, Bangladesh and Pakistan. Among all causal agents of diarrhea, BRV alone is responsible for 62.5% of diarrhea outbreaks in beef and dairy herds. Bovine rotavirus (BRV) is the leading cause of calf diarrhea worldwide. Rotaviruses are recognized as the major causative agent of severe diarrhea in infants and children, and the young of a variety of mammalian and avian species throughout the world. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. The study was funded by Bangladesh Agricultural University Research System. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper and its Supporting information files.įunding: MMA received fund to conduct this study. Received: OctoAccepted: FebruPublished: February 25, 2022Ĭopyright: © 2022 Uddin Ahmed et al. PLoS ONE 17(2):Įditor: Vibhav Gautam, Banaras Hindu University, INDIA (2022) Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh. The predominant genotype could be utilized as the future vaccine candidate or vaccines with the dominant genotype should be used to control BRV diarrhea in Bangladesh.Ĭitation: Uddin Ahmed N, Khair A, Hassan J, Khan MAHNA, Rahman AKMA, Hoque W, et al. To control disease, high-risk areas and younger crossbred calves should be targeted for surveillance and management. ![]() About 23% of calf diarrhea cases were associated with BRV. ![]() The most frequently identified BRV genotype was G6P. The VP4 gene of all P strains showed >90% identities to each other and also with Indian strains. The BRV G6 strains were found to be closest (98.9–99.9%) to Indian strains, and BRV G10 strains showed 99.9% identities with Indian strain. G6P was the predominant genotype (94.4%), followed by G10P (5.6%). Moreover, the risk of BRV infection was 2.6-times (95% CI:1.1–5.8) higher in crossbred (Holstein Friesian, Shahiwal) than indigenous calves. The risk of BRV infection was 3.1-times (95% CI: 1.5–6.5) higher in calves aged ≤ 5 weeks than those aged >5 weeks. The odds of BRV infection were 3.8- (95% confidence interval : 1.0–14.7) and 3.9-times (95% CI:1.1–14.2) higher in Barisal and Madaripur districts, respectively, than Sirrajganj. About 23% of the diarrheic calves were BRV positive. A phylogenetic tree was constructed based on the neighbor-joining method using CLC sequence viewer 8.0. The G and P genotypes of BRV were determined by RT-PCR and sequencing. Risk factors were identified by multivariable logistic regression analysis. The RTSBK positive samples were further tested by polyacrylamide gel electrophoresis and the silver staining technique to detect rotavirus dsRNA. These samples were screened to detect the presence of BRV using rapid test-strips BIO K 152 (RTSBK). Fecal samples were collected from 200 diarrheic calves in three districts between January 2014 and October 2015. In this study we aimed to identify risk factors for BRV infection and determine the G and P genotypes of BRV strains in diarrheic calves. Bovine rotavirus (BRV) is considered the leading cause of calf diarrhea worldwide, including Bangladesh. ![]()
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